# Batch integration feature

Removing batch effects while preserving biological variation (feature output)

3 datasets · 18 methods · 7 control methods · 11 metrics

Task info Method info Metric info Dataset info Results

This is a sub-task of the overall batch integration task. Batch (or data) integration integrates datasets across batches that arise from various biological and technical sources. Methods that integrate batches typically have three different types of output: a corrected feature matrix, a joint embedding across batches, and/or an integrated cell-cell similarity graph (e.g., a kNN graph). This sub-task focuses on all methods that can output feature matrices. Other sub-tasks for batch integration can be found for:

- graphs, and
- embeddings

This sub-task was taken from a benchmarking study of data integration methods.

## Summary

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## Results

Results table of the scores per method, dataset and metric (after scaling). Use the filters to make a custom subselection of methods and datasets. The “Overall mean” dataset is the mean value across all datasets.

## Dataset info

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### Immune (by batch)

Human immune cells from peripheral blood and bone marrow taken from 5 datasets comprising 10 batches across technologies (10X, Smart-seq2) (Luecken et al. 2021).

### Lung (Viera Braga et al.)

Human lung scRNA-seq data from 3 datasets with 32,472 cells. From Vieira Braga et al. Technologies: 10X and Drop-seq (Luecken et al. 2021).

### Pancreas (by batch)

Human pancreatic islet scRNA-seq data from 6 datasets across technologies (CEL-seq, CEL-seq2, Smart-seq2, inDrop, Fluidigm C1, and SMARTER-seq) (Luecken et al. 2021).

## Method info

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### Combat (full/scaled)

ComBat uses an Empirical Bayes (EB) approach to correct for batch effects. It estimates batch-specific parameters by pooling information across genes in each batch and shrinks the estimates towards the overall mean of the batch effect estimates across all genes. These parameters are then used to adjust the data for batch effects, leading to more accurate and reproducible results (Johnson, Li, and Rabinovic 2006). Links: Docs.

### Combat (full/unscaled)

ComBat uses an Empirical Bayes (EB) approach to correct for batch effects. It estimates batch-specific parameters by pooling information across genes in each batch and shrinks the estimates towards the overall mean of the batch effect estimates across all genes. These parameters are then used to adjust the data for batch effects, leading to more accurate and reproducible results (Johnson, Li, and Rabinovic 2006). Links: Docs.

### Combat (hvg/scaled)

ComBat uses an Empirical Bayes (EB) approach to correct for batch effects. It estimates batch-specific parameters by pooling information across genes in each batch and shrinks the estimates towards the overall mean of the batch effect estimates across all genes. These parameters are then used to adjust the data for batch effects, leading to more accurate and reproducible results (Johnson, Li, and Rabinovic 2006). Links: Docs.

### Combat (hvg/unscaled)

### FastMNN feature (full/scaled)

fastMNN performs a multi-sample PCA to reduce dimensionality, identifying MNN paris in the low-dimensional space, and then correcting the target batch towards the reference using locally weighted correction vectors. The corrected target batch is then merged with the reference. The process is repeated with the next target batch except for the PCA step (Lun 2019). Links: Docs.

### FastMNN feature (full/unscaled)

fastMNN performs a multi-sample PCA to reduce dimensionality, identifying MNN paris in the low-dimensional space, and then correcting the target batch towards the reference using locally weighted correction vectors. The corrected target batch is then merged with the reference. The process is repeated with the next target batch except for the PCA step (Lun 2019). Links: Docs.

### FastMNN feature (hvg/scaled)

fastMNN performs a multi-sample PCA to reduce dimensionality, identifying MNN paris in the low-dimensional space, and then correcting the target batch towards the reference using locally weighted correction vectors. The corrected target batch is then merged with the reference. The process is repeated with the next target batch except for the PCA step (Lun 2019). Links: Docs.

### FastMNN feature (hvg/unscaled)

### MNN (full/scaled)

MNN first detects mutual nearest neighbours in two of the batches and infers a projection of the second onto the first batch. After that, additional batches are added iteratively (Haghverdi et al. 2018). Links: Docs.

### MNN (full/unscaled)

MNN first detects mutual nearest neighbours in two of the batches and infers a projection of the second onto the first batch. After that, additional batches are added iteratively (Haghverdi et al. 2018). Links: Docs.

### MNN (hvg/scaled)

MNN first detects mutual nearest neighbours in two of the batches and infers a projection of the second onto the first batch. After that, additional batches are added iteratively (Haghverdi et al. 2018). Links: Docs.

### MNN (hvg/unscaled)

### SCALEX (full)

SCALEX is a method for integrating heterogeneous single-cell data online using a VAE framework. Its generalised encoder disentangles batch-related components from batch-invariant biological components, which are then projected into a common cell-embedding space (Xiong et al. 2022). Links: Docs.

### SCALEX (hvg)

SCALEX is a method for integrating heterogeneous single-cell data online using a VAE framework. Its generalised encoder disentangles batch-related components from batch-invariant biological components, which are then projected into a common cell-embedding space (Xiong et al. 2022). Links: Docs.

### Scanorama gene output (full/scaled)

Scanorama is an extension of the MNN method. Other then MNN, it finds mutual nearest neighbours over all batches and embeds observations into a joint hyperplane (Hie, Bryson, and Berger 2019). Links: Docs.

### Scanorama gene output (full/unscaled)

Scanorama is an extension of the MNN method. Other then MNN, it finds mutual nearest neighbours over all batches and embeds observations into a joint hyperplane (Hie, Bryson, and Berger 2019). Links: Docs.

### Scanorama gene output (hvg/scaled)

Scanorama is an extension of the MNN method. Other then MNN, it finds mutual nearest neighbours over all batches and embeds observations into a joint hyperplane (Hie, Bryson, and Berger 2019). Links: Docs.

### Scanorama gene output (hvg/unscaled)

## Control method info

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### Random Integration by Batch

Feature values, embedding coordinates, and graph connectivity are all randomly permuted within each batch label

### Random Embedding by Celltype

Cells are embedded as a one-hot encoding of celltype labels

### Random Graph by Celltype

Cells are embedded as a one-hot encoding of celltype labels. A graph is then built on this embedding

### Random Integration by Celltype

Feature values, embedding coordinates, and graph connectivity are all randomly permuted within each celltype label

### No Integration

Cells are embedded by PCA on the unintegrated data. A graph is built on this PCA embedding

### No Integration by Batch

Cells are embedded by computing PCA independently on each batch

### Random Integration

Feature values, embedding coordinates, and graph connectivity are all randomly permuted

## Metric info

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### ARI

ARI (Adjusted Rand Index) compares the overlap of two clusterings. It considers both correct clustering overlaps while also counting correct disagreements between two clustering (Luecken et al. 2021).

### Cell Cycle Score

The cell-cycle conservation score evaluates how well the cell-cycle effect can be captured before and after integration (Luecken et al. 2021).

### Graph connectivity

The graph connectivity metric assesses whether the kNN graph representation, G, of the integrated data connects all cells with the same cell identity label (Luecken et al. 2021).

### HVG conservation

This metric computes the average percentage of overlapping highly variable genes per batch before and after integration (Luecken et al. 2021).

### Isolated label F1

Isolated cell labels are identified as the labels present in the least number of batches in the integration task. The score evaluates how well these isolated labels separate from other cell identities based on clustering (Luecken et al. 2021).

### Isolated label Silhouette

This score evaluates the compactness for the label(s) that is(are) shared by fewest batches. It indicates how well rare cell types can be preserved after integration (Luecken et al. 2021).

### kBET

kBET determines whether the label composition of a k nearest neighborhood of a cell is similar to the expected (global) label composition. The test is repeated for a random subset of cells, and the results are summarized as a rejection rate over all tested neighborhoods (Büttner et al. 2018).

### NMI

NMI compares the overlap of two clusterings. We used NMI to compare the cell-type labels with Louvain clusters computed on the integrated dataset (Luecken et al. 2021).

### PC Regression

This compares the explained variance by batch before and after integration. It returns a score between 0 and 1 (scaled=True) with 0 if the variance contribution hasn’t changed. The larger the score, the more different the variance contributions are before and after integration (Luecken et al. 2021).

### Silhouette

The absolute silhouette with is computed on cell identity labels, measuring their compactness (Luecken et al. 2021).

### Batch ASW

The absolute silhouette width is computed over batch labels per cell. As 0 then indicates that batches are well mixed and any deviation from 0 indicates a batch effect, we use the 1-abs(ASW) to map the score to the scale [0;1] (Luecken et al. 2021).

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## References

*Nature Methods*16 (1): 43–49. https://doi.org/10.1038/s41592-018-0254-1.

*Nature Biotechnology*36 (5): 421–27. https://doi.org/10.1038/nbt.4091.

*Nature Biotechnology*37 (6): 685–91. https://doi.org/10.1038/s41587-019-0113-3.

*Biostatistics*8 (1): 118–27. https://doi.org/10.1093/biostatistics/kxj037.

*Nature Methods*19 (1): 41–50. https://doi.org/10.1038/s41592-021-01336-8.

*Nature Communications*13 (1). https://doi.org/10.1038/s41467-022-33758-z.