10X Visium - Mouse Brain Coronal
Gene expression library of Mouse Brain (CytAssist FFPE) using the Mouse Whole Transcriptome Probe Set
Description
FFPE Mouse Brain tissue blocks sectioned as described in Visium CytAssist Spatial Gene Expression for FFPE - Tissue Preparation Guide Demonstrated Protocol. The H&E stained glass slide with tissue section was processed via Visium CytAssist instrument to transfer analytes to a Visium CytAssist Spatial Gene Expression slide. The probe extension and library construction steps follow the standard Visium for FFPE workflow outside of the instrument. The H&E image was acquired using Olympus VS200 Slide Scanning Microscope. Sequencing depth was 53,497 reads per spot. Sequencing configuration: 28bp read 1 (16bp Visium spatial barcode, 12bp UMI), 90bp read 2 (transcript), 10bp i7 sample barcode and 10bp i5 sample barcode. Key metrics include: 2,310 spots detected under tissue; 6,736 median genes per spot; 24,862 median UMI counts per spot.
Preview
An AnnData object with
n_obs × n_vars = 2,310 × 13,985 with slots:
Data structure
| Name | Description | Type | Data type | Size |
|---|---|---|---|---|
| var | ||||
feature_id | Unique identifier for the feature, usually a ENSEMBL gene id. | vector | object | 13985 |
feature_name | A human-readable name for the feature, usually a gene symbol. | vector | object | 13985 |
| layers | ||||
counts | Raw counts | sparsematrix | float32 | 2310 × 13985 |
| uns | ||||
dataset_description | Long description of the dataset. | atomic | str | 1 |
dataset_id | A unique identifier for the dataset. This is different from the `obs.dataset_id` field, which is the identifier for the dataset from which the cell data is derived. | atomic | str | 1 |
dataset_name | A human-readable name for the dataset. | atomic | str | 1 |
dataset_organism | The organism of the sample in the dataset. | atomic | str | 1 |
dataset_reference | Bibtex reference of the paper in which the dataset was published. | atomic | str | 1 |
dataset_summary | Short description of the dataset. | atomic | str | 1 |
dataset_url | Link to the original source of the dataset. | atomic | str | 1 |
Download & explore
The processed dataset lives on the OpenProblems public S3 bucket, which is world-readable. Download the file directly, or copy its S3 URI to fetch it with your tool of choice.
s3://openproblems-data/resources/datasets/tenx_visium/visium/mouse_brain_coronal/dataset.h5ad Used in
References
- 10x Genomics. (2022). Mouse brain coronal section 1 (FFPE). link ↗